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Vitamin D deficiency correlates with a substantial decline in physical performance (4). Common clinical manifestations of vitamin D deficiency in relation to muscle include symmetric low back pain, proximal muscle weakness, and muscle aches (2, 3). Drew University of Medicine and Science, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California 90095Department of Internal Medicine (L. The merge image was obtained by fusing the green (FITC) and the blue (DAPI) filters pictures.
Fields were photographed with a SPOT-RT digital camera and acquisition software (Diagnostic Instruments, Sterling Heights, MI). Drew University of Medicine and Science, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California 90095Department of Internal Medicine (L. Furthermore, mice lacking the VDR show a skeletal muscle phenotype with smaller and variable muscle fibers and persistence of immature muscle gene expression during adult life, suggesting a role of vitamin D in muscle development (12, 13). It has been reported that 1,25-dihydroxyvitamin D (1,25-D3) induces genomic effects, leading to the synthesis of new proteins that affect muscle cell contractility, proliferation, and differentiation (11). The mouse growth factor RT Profiler PCR array contains genes related to growth factors that play vital roles in various normal biological processes, such as embryogenesis, wound healing, and inflammation. Total RNA aliquots were converted by RT, and the resulting c DNA were subjected to the Mouse Growth Factor (PAMM-041) and the Mouse Skeletal Muscle: Myogenesis & Myopathy (PAMM-099) PCR arrays (SABiosciences Corp.).